Direct Intra Peritoneal Insemination (DIPI)

Direct intraperitoneal insemination (DIPI) is one of the least invasive strategies of assisted reproduction. Unexplained infertility, cervical factors, and male sub-fertility are major indications for DIPI.

DIPI is usually performed 36 hours after trigger injection, and confirming ovulation through an ultrasound scan. For DIPI, the partner's semen was obtained by masturbation and liquefied at room temperature and then its amount, sperm concentration, and sperm motility were evaluated. The vagina will be cleansed and TV USG will be performed to identify the fluid in the posterior cul-de-sac (PCDS) and ovaries. A 30 cm long 17 gauge disposable needle (Cook) will be placed in a needle guide attached to the vaginal probe. The needle will be put into the PCDS along with the biopsy guide. The syringe containing the prepared semen sample is attached to the needle and the semen sample of 2 mL will be injected directly into the PCDS.

DIPI was particularly effective in cases of infertility due to cervical mucus insufficiency and unexplained infertility with results respectively of 33.7% and 30.4% per couple and 30.7% and 28.7% per DIPI cycle.